ABSTRACT
Objective To explore the effect of enriched environment on neuroregeneration in subventricle zone(SVZ)of rats with vascu-lar dementia.Methods All rats were divided into sham group(n=5),vascular dementia group(VD group,n=12)and enriched environment group(EE group,n=12).Then,2-VO method was applied to make the vascular dementia model.The sham group and VD group received conventional breeding environment for 30 days,while EE group was subjected to enriched environment for 30 days.Immunohistochemistry was applied to detect the BrdU and Doublecortin(DCX)expression in SVZ,and DCX/Ki67,DCX/p-cAMP-response element binding pro-tein(CREB)expression in SVZ was assessed by immunofluorescence double-labeling technique.Results Compared with the sham group, the number of DCX+cells(t=2.989,P=0.026)and BrdU+cells(t=3.069,P=0.005)increased in VD group,and increased more in EE group (t=3.067, P=0.027; t=2.907, P=0.011). Besides, the number of the DCX/Ki67 (t=2.994, P=0.040) and DCX/p-CREB (t=4.707, P=0.009) cells was significantly higher in EE group than in VD group.Conclusion Enriched environment could up-regulate the neuroregeneration ca-pacity in SVZ of rats with vascular dementia through CREB signal pathway.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of Kudou Shencha decotion on INF-y, ICAM-1, MCP-1 levels of prostate tissue homogenate in immunity prostatitis model rats.</p><p><b>METHOD</b>Forty Wistar male rats were divided into 5 groups randomly: Kudou Shencha decotion group with high dosage and low dosage, Qianleitai group, the model control group and normal group. The rat model of chronic nonbacterial prostatitis was established by multiple hypodermical injection of the suspension of prostatic protein purification with Freund's completed adjuvant. The level of intercellular adhesion molecule (ICAM-1), interferon gamma (INF-gamma) and monocyte chemotactic protein-1 (MCP-1) were measured by enzyme linked immunosorbent assay (ELISA).</p><p><b>RESULT</b>The content of ICAM-1 and MCP-1 in the model group was higher than that of the normal group (P < 0.05), the content of ICAM-1 was obviously decreased in Kudou Shencha decotion group with high dosage (P <0.05), the contents of MCP-1 were all obviously decreased in Kudou Shencha decotion groups and Qianlietai group. Compared with the model group, the contents of INF-gamma in all treatment groups were decreased insignificantly.</p><p><b>CONCLUSION</b>Kudou Shencha decotion has the action of lowering the level of ICAM-1 and MCP-1, which may be one of the mechanisms of Kudou Shencha decotion in the therapy of chronic prostatitis.</p>
Subject(s)
Animals , Humans , Male , Rats , Chemokine CCL2 , Metabolism , Drugs, Chinese Herbal , Intercellular Adhesion Molecule-1 , Metabolism , Interferon-gamma , Metabolism , Prostate , Metabolism , Prostatitis , Drug Therapy , Metabolism , Rats, WistarABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Hewei Xiaopi Capsule (HXC) in treating patients with dyskinesis functional dyspepsia (FD).</p><p><b>METHODS</b>Sixty-three patients with FD were randomly assigned to two groups, 33 in the treated group and 30 in the control group, who were treated respectively with HXC and domperidone for 4 weeks to observe the clinical effect and changes in electrogastrogram (EGG) before and after treatment.</p><p><b>RESULTS</b>Symptoms were alleviated in both groups after treatment, 3 patients in the treated group were cured, the efficacy was judged as markedly effective in 11, effective in 14 and ineffective in 5, while in the control group, 1 cured, 8 markedly effective, 14 effective and 7 ineffective, difference between the two groups showed no statistical significance. EGG showed that in the treated group, the normal slow-wave percentage was 41.93 +/- 18.22 before treatment and 50.86 +/- 16.03 after treatment, showing significant difference (P < 0.05).</p><p><b>CONCLUSION</b>HXC is markedly effective in treating patients with FD.</p>